Journal: ACR Open Rheumatology
Article Title: Distinct DNA Methylation Patterns of Rheumatoid Arthritis Peripheral Blood and Synovial Tissue T Cells
doi: 10.1002/acr2.11231
Figure Lengend Snippet: Principal component analysis (PCA) and hierarchical clustering of rheumatoid arthritis (RA) and osteoarthritis (OA) peripheral blood and synovial CD3 + T cells based on differentially methylated loci (DML). The separation between peripheral blood and synovial tissue is shown in RA ( A ) and OA ( B ) samples. Comparisons between RA and OA samples are shown in peripheral blood ( C ) and synovial tissue ( D ), with a less stringent cutoff. The arrows indicate the two arthroplasty samples from the same patient. E , Hierarchical clustering by using combined DML separates four categories of samples. Dashed lines connect samples in hierarchical clustering and the corresponding PCA plot. PC, principal component.
Article Snippet: T cells were isolated by using anti‐CD3 conjugated magnetic bead separation (EasySep CD3 Positive Selection Kit; STEMCELL Technologies Inc).
Techniques: Methylation